Localization of Concanavalin A Binding Sites with a Concanavalin A-Alkaline Phosphatase Method

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in a series of different vertebrate tissues, concanavalin A binding sites were localized precisely by an alkaline phosphatase-labelled concanavalin A-diazo blue B (Con A-ALP-DBB) method. The results obtained with control staining procedures for the Con A-ALP-DBB method substantiated the usefulness of this staining method. The successful use of Con A-ALP conjugate in the Con A-ALP-DBB staining method is regarded as a starting point for the development of Con A-ALP methods which are better than Con A-peroxidase (PO) methods in the preservation of tissue sections and are applicable to tissue specimens for both light and electron microscopy.