Abstract
Phospholipase D (PLD)-mediated transphosphatidylation enables enzymatic syntheses of phospholipids having various head groups. Due to the broad substrate specificity as well as the ease of preparation, PLDs of Streptomyces origins have been widely used for phospholipid production. For a long time, however, synthesis of phosphatidylinositol (PI) has not been realized with Streptomyces PLDs. The author’s research group has developed mutant PLDs having PI-synthesizing activity by protein engineering technique. This review introduces our recent results for the development of PI-synthesizing PLD and its application.
