Abstract
In this screening study, we report the characteristics and functions of the following novel enzymes isolated from actinomycetes: phospholipase (PL) A1, PLB, PLD, and glycerophosphocholine cholinephosphodiesterase. Investigations revealed that the characteristics of these enzymes differed from those of known phospholipases. These novel enzymes could have valuable applications as industrial enzymes, in material production, and in diagnostics. In this study, we produced a biodiesel fuel and glycero-3-phosphocholine by using PLA1 or PLB. In addition, PLA1 hydrolyzed the sn-2 acyl ester bond of phosphatidylethanolamine plasmalogen, indicating the potential for use as a diagnostic enzyme. We also observed novel substrate specificity for PLD and determined that phosphatidylcholine plasmalogen could be analyzed using PLB and PLD.
