2016 Volume 16 Issue 5 Pages 213-224
Chiral-phase HPLC has been widely used as an essential means of separating and quantifying enantiomers in biological and synthetic samples. The methodology has been extended to the direct resolution of various synthetic and naturally occurring chiral glycerolipids, such as triacylglycerols and phospholipids, since the enantiomer resolution of synthetic mono- and diacylglycerols as 3,5-dinitrophenylurethane derivatives was achieved by chiral-phase HPLC in the 1980s. This review describes recent advances and applications of chiral-phase HPLC in lipid research. In particular it is focused on the analysis of enantiomeric glycerolipids including triacylglycerols and phospholipids.
