2017 Volume 17 Issue 3 Pages 117-125
Accumulation of carbon dioxide by fossil fuel has caused global warming. Biodiesel fuel by plant lipid esterification is used for inhibiting the carbon dioxide increased. It is necessary that plant lipid can be obtained with microorganism on different environment from plant, because global warming has a bad influence upon agriculture. A new method for Lipomyces screening of neutral lipid high accumulating ability was performed with many number combinations of renewable carbon sources and strains. The new method for estimating lipid accumulation ability consists of plate cultures, micrographs and measurements of the volume of lipid globule, which is a morphological feature of Lipomyces. Yeast strain of the high accumulating ability has high neutral lipid conversion efficiency and has leakage ratio of neutral lipid from lipid globule. Methods for breakdown of lipid globule membrane and cell membrane for efficient recovery of lipid globule neutral lipid were examined. Trends in microbial lipid production research are also reviewed.
