Determination of Phospholipids Quantity Using NMR Spectroscopy

Abstract

NMR spectroscopy is a widely used method in study and analysis of phospholipids. Proton, carbon and phosphorus nuclei are commonly used in the NMR measurements for phospholipids. For use as a quantitative test, ³¹P NMR, which is observable by separating phospholipids into individual classes, is particularly important. The study of phospholipid by employing ³¹P NMR has a history of 50 years, and has already accumulated a lot of knowledge. We achieved accurate, individual and concomitant quantifications of phospholipid classes (mainly glycerophospholipids) in commercial polar lipids by using the ³¹P NMR technique incorporating a ¹H NMR methodology which is becoming popular as a method to determine the purity of low molecular organic standard substance. In this review, we describe about phospholipids quantification by using ³¹P NMR based on our previous study results.