Practical Guide for Lipidomics

Abstract

Lipids are the main component of cellular membranes, function as energy storehouses and as important signaling molecules. Thus, alterations of membrane lipid homeostasis are linked to various diseases. Lipidomics is the large-scale study of the lipid produced in all living organisms and examine the structures, functions, interactions, dynamics of cellular lipids in the biological system. Lipidomics has been driven by rapid advances in technologies such as various chromatography technologies, mass spectrometry (MS), enzymatic fluorometric assays and protein probe methods. On the other hand, lipids are metabolically and chemically unstable, data errors and artifacts will occur without the accurate knowledge and techniques of analysis. In this review, I will share the important points of the work process of lipidomics based on MS technology and their experimental example.