Detection and Structural Analysis of Lipid-derived Radicals and Oxidized Lipids and Their Application to Disease Models

Abstract

Recently, lipid-derived radicals and oxidized lipids have been reported to play critical roles in cell death, particularly ferroptosis, as well as in inflammatory responses, and have therefore attracted considerable attention. However, because of their extremely high reactivity, techniques for accurately measuring these species have been limited. To accelerate research in this field, direct detection of these molecules is indispensable. Furthermore, the development of appropriate inhibitors will also be required. In this article, we describe methods for the detection and structural analysis of lipid-derived radicals, which serve as the chain reaction centers of lipid peroxidation, as well as analytical techniques for elucidating the structures of oxidized phospholipids.

graphical abstract