Abstract
The purpose of this study was to investigate the behavior of auto-transplanted cultured periosteum (CP) cells from rats. CP cells were obtained from the calvarium, and bone marrow cells were obtained from the femur. Cell proliferation rates and expression of bone-related protein mRNAs were evaluated. CP and cultured bone marrow (CBM) cells were auto-transplanted into the rectus abdominal muscle and also into the bone cavity of femurs. Immunohistochemical staining with PEBP2αA was performed. Moreover, the expression of mRNAs was also analyzed. Cultured gingival fibrous (CGF) cells were used as controls. The proliferation ratio of CP was significantly higher at 3, 5 and 7 days compared with CBM. Ectopic bone formation in the muscle was observed at 7 days after the CP transplantation. However, little bone formation was observed at 14 days in CBM transplanted areas. PEBP2αA-positive cells in the bone cavity were observed in the CP transplanted group at day 3 and newly-formed bone was seen at 7 day. Th e expression of bone-related protein mRNAs of CP were detected earlier than in any of the other groups. CP has high proliferation potency and can differentiate more quickly than CBM. Thus, CP is the most suitable osteogenic tissue to serve as graft material.
