抄録
The purpose of this study was to evaluate the responses of immature young pulp cells under conditions of hypoxia with or without reoxygenation. Proliferative activity and bone related protein mRNAs were evaluated. Proliferation ratios in the hypoxia only and hypoxia with reoxygenation groups were significantly lower than those in the control group on days 2 and 3. On day 4, there was no significant difference between the control and the reoxygenation group. There was no significant difference in alkaline phosphatase (ALP) mRNA among the control, hypoxia and reoxygenation groups at any of the time periods. Expression of dentinsialophosphoprotein (DSPP) mRNA was significantly lower in the hypoxia group than in the control and the reoxygenation group on days 2 and 3. Hypoxic condition suppressed the expression of a gene specific to odontoblasts in immature young pulp cells, suggesting that it affects the differentiation ability of odontoblasts.
