Molecular Mechanisms of Inhibiting the Muscle Atrophy by Electroacupuncture

Abstract

 Electroacupuncture (EA) is an acupuncture technique that is stimulated by acupuncture needles with low-frequency microcurrent. The aim of this study is to elucidate the effect of EA and it's molecular mechanism on muscle atrophy by using an animal model: hindlimb-suspended (HS) mice in the disuse muscle atrophy model. To compare the effects of EA in HS mice and HS mice treated with EA (EA/HS), soleus muscle mass and soleus myofiber diameter were measured. We then used real-time quantitative RT-PCR to analyze the expression of myostatin and ubiquitin ligase genes in atrophic muscles of HS mice and in muscles of EA/HS mice. We found that EA/HS mice maintained a soleus muscle mass that was not significantly different from that of wild mice (WT), whereas HS mice had significantly reduced muscle mass. Also, the diameters of myofibers in EA/HS mice, which were not significantly different from wild values, were significantly larger than those in HS mice. Repeated EA treatment suppressed gene expression of myostatin and ubiquitin ligase genes in skeletal muscle of EA/HS mice but induced expression of these genes in HS mice. These findings suggest the molecular mechanism by EA: suppression of myostatin and ubiquitin ligase gene may be a key reaction of inhibiting the disuse muscle atrophy.