Optical Review
Print ISSN : 1340-6000
ISSN-L : 1340-6000
Observation of Subcellular Structures of Neurons by an Illumination Mode Near-Field Optical Microscope under an Optical Feedback Control*
Rajagopalan UMA MAHESWARI, Shuji MONONOBE, Hitoshi TATSUMI, Yoshifumi KATAYAMA, Motoichi OHTSU
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1996 年 3 巻 6B 号 p. 463-467

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We propose an illumination mode near-field optical microscope operated under an optical feedback for imaging biological specimens in their natural environment. For feedback control, rapidly varying evanescent signal has been generated over the sample surface. It has been found that evanescent signal can be profitably used as a control signal by utilizing its sample feature dependent discrimination sensitivity. Neurons have been successfully observed both in air and in liquid with a resolution well beyond the diffraction limit.
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© 1996 by the Optical Society of Japan
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