生体イメージング法による細胞・臓器のリアルタイム評価法の開発

抄録

The organ function and its regulatory mechanisms which are affected by various physiopathological conditions have been analyzed by molecular and biochemical methods. We have been working on developing various optical probes to visualize cellular and organ conditions/functions in vivo. An innovative technology is essential for imaging the biological functions, especially by using bioluminescence；for example, a novel highly transmittable and sensitive optic probe. We have, so far, developed some novel probes based on the unique bioluminescence theories. By applying these probes for biological systems, we succeeded to visualize the dynamic changes of liver injury and oxidative stress in mouse liver ischemia/reperfusion model by the caspase-3 activity probe and redox sensitive GFP probe, respectively. These novel bio-imaging techniques will definitely bring great possibility and potential impact for research and clinical medicine in the future.