Preparation of cells from surplus tissues of hyperdactylia.

Abstract

Here we introduce the protocol for cell cultures of various types of cells from surplus tissues of hyperdactylia. The remnant polydactylous finger tissues arose by surgeries were obtained from the medical organization in Japan under the strict supervision of institutional research review board in accordance with ethical guidelines. The samples (skin, adipose, bone, etc.) were provided as fresh tissues or frozen samples immersed in cryoprotectant. The skin pieces were digested with dispase, and were separated into epidermis and dermis. From epidermal tissues treated with trypsin, keratinocyte and melanocyte cultures were established. From dermis, fibroblasts were obtained ether by collagenase treatment and by explant cultures. In addition, keratinocytes intermingling in dermal cultures could be isolated. The adipose tissues were treated with collagenase, and preadipocyte cultures can be obtained. We also cultivated bone-derived cells. Each cell culture was confirmed to exhibit the specific characteristics of each cell type. Thus surplus tissues of hyperdactylia are potential bioresources to establish cultures of various cell types for life science researches.