Abstract
In this review, the progress and development of regenerative medicine, therapy with cultured cells is shown. We have developed temperature-responsive culture dishes. Since a temperature-responsive polymer, poly (N-isopropylacrylamide) is covalently immobilized on the surfaces, upon temperature reduction below 32°C without need for any proteolytic enzyme, cultured cells are noninvasively harvested as a contiguous cell sheets together with the own extracellular matrix deposited during culture beneath the cell sheets. In particular, clinical applications of cell sheet-based regenerative medicine in various fields that we have performed were focused on.
