光技術を用いた臓器・細胞機能評価と臨床応用

抄録

To develop an effective organ/cell preservation method and to monitor post-transplant graft function continuously and non-invasively, an innovative optic technology to visualize cell/organ function seems to be useful. We have developed some optic probes to visualize regulated cell death (apoptosis, necroptosis, pyroptosis), redox states and cellular stresses, pH and cellular antigens in deeper lesions of the organ. In the hepatic ischemia/reperfusion model of mice, we successfully imaged liver oxidative stress (by redox-sensitive GFP) and apoptosis (by caspase-3 activity) non-invasively and chronologically in a single mouse. We also developed a unique tool to visualize intracellular pH and succeeded in imaging dynamic changes of pH in a mouse posterior limb ischemia/reperfusion model. We are also developing the new devices for tissue/organ imaging. We are trying to monitor the optic signals chronologically and track the lesions in the body by developing light sensor and multiple CCD camera system, which can be used in endoscopic examination and surgical operation. It is still on a way, but this kind of technology will definitely provide a new avenue toward effective and non-invasive surgical therapy in the future.