Amniotic epithelial cells (AECs) have garnered significant interest for use in regenerative medicine due to promising features, such as multipotency, low immunogenicity, non-tumorigenicity, and anti-inflammatory capacity. AEC transplantation has been attempted as an alternative to hepatocyte transplantation for the treatment of certain liver diseases. Although intra-portal infusion has been considered as one of the leading candidates of transplantation methods in clinical practice, the protocol of intra-portal AEC administration has not been fully investigated. Thus, in order to clinically introduce new cell transplantation treatment using AECs, an optimal cell administration protocol needs to be established.
