Abstract
Objectives: To examine the role of CysLTs in CRS, nasal polyp specimens were obtained from patients with CRS classified into 3 subgroups: those with aspirin-intolerant asthma (CRS-AIA group), those with bronchial asthma (CRS-BA group), and those without asthma (CRS-NA group).
Design: Samples were prepared from the nasal polyps of patients undergoing endoscopic sinus surgery (ESS) at our hospital. The CysLTs were extracted using the C18 Sep-Pak column (Waters Corp, Milford, MA) and quantified using a high-sensitivity competitive EIA kit (Cayman chemical, Ann Arbor, MI) in accordance with the manufacturer's instructions. The expressions of the CysLT1 and CysLT2 receptors in the nasal polyp specimens were evaluated by immunohistochemistry.
Subjects: Twenty-seven patients who had undergone ESS.
Results: The concentrations of the CysLTs were significantly higher in the polyps obtained from the CRS-AIA group than in those obtained from the CRS-BA or CRS-NA group. There were no significant differences in the nasal polyp CysLT concentrations between the CRS-BA group and CRS-NA group. Expressions of both the CysLT1 and CysLT2 receptors were significantly more intense? stronger? higher? in the specimens from the CRS-AIA group than in those from either of the other two groups, while there were no significant differences in the expression levels of the CysLT1 and CysLT2 receptors in the nasal mucosal inflammatory cells.
Conclusions: These results suggest that CysLTs may play prominent roles in the pathogenesis of aspirin-intolerant asthma associated with CRS.
