Nalidixic Acid類縁抗菌剤の蝸牛分布に関する実験的研究
とくに凍結マクロオートラジオグラフィー法の応用について
1982 年 13 巻 1 号 p. 141-144
詳細
1982 年 13 巻 1 号 p. 141-144
The distribution of three nalidixic acid analogs (14C-Pipemidic acid, 14C-AM-715 and 14C-OPC-7241) to cochlea in rabbits was studied by macro-autoradiography and radiometry. Macro-autoradiography was performed 30 minutes after intravenous administration of 14C-labelled antimicrobic drugs in about 80μCi/12mg/kg. Radiometry was performed 30 minutes, 1 hour and 3 hours after intravenous administration of three drugs in about 20μCi/10mg/kg. Horizontal frozen sections parallel to mandibular basis were cut in 50μm thickness in a cryostadt. In autoradiograms, the blackening in cochlea is not so significant. Quantitatively, levels of 14C-labelled three drugs radioactivity in cochlea were low. Compared the above results, macro-autoradiography can be one of the useful means to evaluate the distribution of chemotherapeutic agents to cochlea.
