フロセマイドとEP

抄録

Furosemide, as a loop diuretic, causes a transient decrease of endocochlear potential (EP) in guinea pig. Many parameters, such as maximal decrease percentage of EP etc., have been tried to analyse the decrease of EP caused by furosemide. In cochlea, furosemide may interfere with a enzyme process which is a part of EP generation pump. When an analytical method of chemical process is applied to this EP decrease, more qualitative analysis of EP generation is possible. In this experiment, we recorded EP in 42 adult guinea pigs with aid of microelectrode technique in response to furosemide (20mg, 30mg, 40mg, 50mg/Kg). Values of EP decrease to furosemide can be shown by the following experimental equation.
Y=L (1-e^{-r (x-a)})……(1) All of EP responses showed good fitting to the acquired experimental equation. This means that furosemide blocks an enzyme process of EP generation in a way of first order reaction.