顕微蛍光測光法による蝸牛構成細胞の核DNA定量

抄録

In spite of the recent remarkable progress of research in the cochlea, little is known about the kinetics of its component cells. Toelucidate it, Feulgen-DNA cytofluorometry was applied to the cochlea. A piece of Reissner's membrane and a sheet of hair cells free from supporting cells were prepared carefully and spread on a glass slide. For the latter tissue, it was necessary to separate the cells from each other to avoid the interference of fluorescence. The tissues were stained with Azocarmin G and acriflavin-Feulgen. Azocarmin G not only blocked nonspecific fluorescence but also made it posible to distinguish the aimed cells from others. After post-irradiation, the amount of Feulgen-DNA in the cells of Reissner's membrane and that in the hair cells comparing them to that in the cerebellar internal granule neurons. According to the results, all the component cells of Reissner's membrane andthe hair cells appeared to have typical diploid G1 characteristics.