1984 Volume 15 Issue 1 Pages 46-50
Horseradish peroxidase, type IV (HRP) was injected into the tympanic bullae of seven immuninized chinchillas and twenty immuninized guinea pigs, to take part in the Arthus' reaction in the middle ear cavity. Absorbed HRP interacted with IgG and deposited on the basement membrane as immune complexes. But within 24 hours, these immune complexes were clearly removed from the submucosal space by the infiltrated neutrophiles. Since this period, tympanic cavity was filled with effusion containing exudated cells and desquamated cell debris. These effusion also obstructed the lumen of the eustachian tube and stimulated the mucous secretion, that brought the disturbed ciliary transportation. Under these conditions, it took 10-14 days that tympanogram indicated the disappearence of middle ear effusion. After 3 weeks from the first injection, second injection was underwent. At this time, much exaggerated mucosal responce took place, and lympho-plasmacytic infiltration with follicle was observed. Upon these observation, this animal model was quite resemble to the human secretory otitis media on the tympanographical and clinicopathological point of view. In conclusion, the Arthus' reaction was highly suspected to play a role in the pathogenesis of secretory otitis media.
