Pathogenesis of otitis media with effusion in children

Part. 3- Analysis of the chemotactic factor in middle ear effusion

Abstract

The purpose of this work is to examine the etiologic factor of otitis media with effusion, especially in its chronicity of the inflammation in the middle ear. In the last congress, we have reported the relationship between the chemotactic activity and the cytology of middle ear effusions (MEEs). On the bases of these results, we tried to evaluate the factor with chemotactic activity in MEEs, focusing on immune complex. In this study, MEEs were treated as follows:
1) Incubation at 56°C for 30 min, 2) Treatment with Protein A bacterial adsorbent, 3) Treatment with Polyethylenglycol, 4) Treatment with anti C3 and C5 antibodies, and 5) No treatment MEEs. By using Boyden chamber method, we evaluate the chemotactic activities in those treated MEEs.
The result was that MEEs especially with high chemotactic activities were showed marked reduction in their activities. And it is suspected that immune complex may be related to these results.