The distribution of cholin acethyltranseferase-like immunoreactivities (CATI) and glutamic oxaloacetic transaminase-like immunoreactivities (GOTI) were studied immunohistochemically in the auditory and vestibular system of rat brain. CATI neurons were iocated in the superior olivary nuclear complex, the medial vestibular nucleus and the prepositus hypoglossal nucleus. Both cytosolic (s-) and mitochondrial (m-) GOTI neurons and fibers were unevenly distributed in the all auditory and vestibular nuclei. However, the Iatelar vestibular nucleus contained only m-GOTI neurons. These findings suggested that acethylcholine and glutamate/aspartate play important roles in the central auditory and vestibular system.