Vibration mode of the malleus was investigated in nine fresh human temporal bones using a video-measuring system (Technical Instrument Co). The vibration amplitude and phase angle of the umbo and malleus head were measured between 0.1 kHz and 4.5 kHz. The umbo moved piston-like at 0.1-0.8 kHz and 2.6-4.5 kHz but in an ellipse at 1-2.4 kHz. The malleus head showed elliptical movement. The position, displacement and phase angle of the rotation axis of the malleus was calculated based on the displacement and phase angle of the umbo and malleus head. The axis was around the level of the neck in frequencies lower than the resonant frequency, beneath the level of the short process near the resonant frequency and the top of the malleus head in higher frequencies. The average axis displacement was 0.9 microns at 1 kHz.