We examined the biomechanical properties of Bull Frog tympanic membrane under static as well as dynamic pressure conditions, using the manufactured faigue testing machine for biological soft tissue materials. 1. The stress relaxation is the property of a visco-elastic material which we had already shown in the collagen membrane experiment, was confirmed in the relaxation test. 2. In the fatigue test, the applied load contianed to decrease even after the time which no decrease in the applied load was seen in the relaxation test. From the point of the strength of the material, there is a on-off behavior by the degree of the initial load in the relaxation test, while in the fatigue test there is a time dependent deterioration controlled by the cyclic effect.