Mechanical properties of tympanic membranes in guinea pigs and human beings were measured by using the microtension tester. The test was carried out by changing speed (1, 25, 50mm/min), changing tension direction (right angle and parallel to radial fiber). There was no significant difference in the tensile strength by changing tension speed. Referance to tension direction, parallel to radial fiber was 9.1 times stronger in guinea pigs, 1.3 times stronger in human beings than right angle to radial fiber.