We have continued to undertake computer-aided three-dimensional reconstructions of a temporal bone from routinely processed histologic sections. This time, using the upgraded computer system, reconstruction of the membranous labyrinth and the inner ear sensory organs was performed. Owing to the system's “semitransparent display” function, the reconstructed sensory organs could be observed through the simultaneously reconstructed membranous labyrinth. The results were satisfactory for understanding the spatial relationships of these structures. Computer-aided measurements were also attempted, and the length of the organ of Corti and the angle between the saccular and utricular maculae were calculated.