Electron microscopic study was performed on the tympanic plexus taken from patients during ear surgery. The nerve bundles of the plexus consisted of mostly myelinated fibers and unmyelinated fibers distributing among them. Ganglion cells were found along the course of the nerve bundles. Nerve endings, containing many agranular vesicles, formed synaptic contacts with cell bodies of these ganglion cells. As these nerve endings were suggestively cholinergic, ganglion cells were autonomic. We speculate they belong to the parasympathetic system.