The aim of this paper is to elucidate the anatomic variation of the jugular fossa (JF) on the basis of examination 65 human temporal bones. Observations were made on temporal bones sectioned along a plane including the cochlea, the JF, and long axis of the internal canal. The result reveals that 26 out of 65 ears are type I, 29 are type II, and 10 are type III. In 60% of ears the upper margin of the JF higher than the floor of tympanic cavity, and in 89% of ears the former is situated medially in the latter. In conclusion, protrusion of the jugular bulb into the tympanic cavity is not caused by the abnormal higher JF, but by its lateral displacement.