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Service robots are more popular than before. Prior studies related to service robots have mainly examined about appearance, facial expression, voice, gesture and motion. However, it is not clear which characteristics of service robots have an effect on affective interaction most and how they work together. This study focuses on how humans perceive the emotions from the motions of service robots, and how the emotions perceived from the motion of service robots is different from those of human and animation. The experiment was designed with 3 independent variables, such as media (human, animation and robot), motion size (large and small) and motion velocity (fast and slow), and perceived emotions as a dependent variable. 30 participants took part in the experiment, and the results indicate that the small size of motion is preferred than the large size of motion when the motions are performed by animation and actual robots, in terms of ‘favorable’ and ‘enjoyable’emotions. These results provide a guideline for designing the motions of service robots.