サイトカイン濃度勾配下での好中球の偽足を含む膜特性の水中推進機構への影響

抄録

The goals of this study are to examine the propulsion of neutrophils which move in water in the direction of high concentration of the cytokine and to apply it to DDS. Assuming that neutrophils move by the concentration Marangoni effect, we experimentally examined changes of cytokine concentration on the surrounding fluid and neutrophil membrane. But detailed propulsion mechanism is unknown. Especially, the effects of pseudopods which are used when neutrophils move on the inner wall of blood vessels on the underwater propulsion, and the effects of living neutrophils and dead ones on membrane properties are still unknown. Then, in this study, the observation using the microscope was carried out, and the moving speed of neutrophils and the concentration gradient on the membrane was quantified. Besides, the effect of the membrane properties on the propulsion mechanism was investigated by examining the difference of these values living status, dead status, with pseudopods and without pseudopods. The results show that there is a large difference of cytokine concentration on membrane, in case of pseudopods. It was concluded that both neutrophil’s velocity and concentration gradient on the membrane are greatly influenced by living status and dead status.