To well characterize disinfection system and to investigate the mechanism of cell killing by TiO_2/US method, Escherichia coli (E. coli) was used as a model and target microorganism. At present, our research is going on developing disinfection system by using ultrasonic reactor in the presence of nonwoven TiO_2 fabric. It was found that the decrease in cell concentration was almost insignificant without ultrasonic irradiation although TiO_2 filter was present, and vice versa. In contrast, applying ultrasonic irradiation with TiO_2 filter reduces the E. coli cell concentration very rapidly which indicated very high disinfection power at this condition or system. However, the effect of new ultrasonic reactors on the disinfection rate, the characterization of affecting parameters of the ultrasonic reactors, as well as possible germicidal mechanism of disinfection remain to be studied. Given an opportunity to conduct these research will uncover above issues and will be benefited our scientific career to develop, in addition to enrich the bio-industrial field as well.