P11 ベシクル水溶液に対する超音波照射効果の研究(ポスターセッション)

抄録

In order to clarify the effect of sonication to vesicle solution, we performed two series of experiments. Ultrasound frequency we used was 480kHz in both experiments. In the first experiment, we measured fluorescence decay of pyrene dissolved in the aqueous solution of didodecyldimethylammonium bromide (DDAB) under ultrasonic irradiation. It was found that fluorescence decay was promoted by ultrasonic irradiation. Mechanism of quenching by bromine generated from the bromide ion which was the counter ion of DDAB was suggested. It was shown that the suggested mechanism did not contradict with the experiment with additional bromine and quantitative evaluation of the chemical effect by potassium iodide (KI) dosimetry. In the second experiment, we prepared aqueous solution of dilauroyl phosphatidylcholine (DLPC) and homogenized vesicle size by using extruder. Then, we performed ultrasonic irradiation and analyzed the size distribution by dynamic light scattering (DLS). Decrease of size to a diameter around 100nm was observed up to three hours, and substantial increase of size was observed afterwards.