P28 非侵襲刺激応答型ナノキャリアを用いたドラッグデリバリーシステムの構築

抄録

Chemotherapy is often complicated by toxic side-effect of the antitumor drugs. To solve this problem, it is necessary that antitumor drugs are effectively delivered to the tumor site specifically. The drug delivery system (DDS) using liposome as drug carriers has been proposed. However traditional DDS can not achieve full therapeutic effect. Recently, next-generation DDS that combines treatment of utilized physical energy and DDS of antitumor drugs is proposed. Therefore we propose development of pinpoint antitumor drug delivery using ultrasound and liposome as the next-generation DDS. We used liposome modified with thermosensitive polymer as ultrasound stimulus-responsive carrier. This polymer develops phase transition at a lower critical temperature (LCST) and interacts with liposomal membrane. In this study, we made the calcein-loaded thermosensitive liposome (TSP-Lip), and irradiated it with ultrasound. After irradiation, the release of calcein from liposome was measured by the fluorescence intensity with a spectrofluorometer. The results indicated that the release of calcein from TSP-Lip was increased with an increase of ultrasound intensity compared to the non-modified liposome.