Lignocellulose is one of the most promising materials for biofuel production. However, lignin covering cellulose fiber and microcrystalline cellulose and hemicellulose is obstacle against effective saccharification by enzyme. Therefore in the present study, we employed ultrasonic (US) in ionic liquid for simple and effective pretreatment of biomass, which was needed for subsequent enzymatic saccharification. The saccharification ratio enhanced from 18% to 87% by US treatment for 30 min in [Emim OAc]. This might be attributed to that lignin structure depressed under US irradiation, which eanhanced decrystallization of cellulose and hemicellulose. So enzyme acceptable site increased in cellulose. These results suggested IL/US