The effects of ultrasound irradiation on enzymatic hydrolysis of enolesters with and without albumin were examined. In our previous studies, the enzymatic hydrolysis of 2-benzylcyclohex-1-enyl acetate by lipase afforded the corresponding racemic ketone. On the other hand, the reaction with bovine serum albumin proceeded with high enantioselectivity to give the (S)-ketone. In the presense of albumin, enzymatic hydrolysis was accelerated by ultrasonic irradiation. On the contrary, in the absence of albumin, improvement of reactivity could not be observed.