抄録
A system for the determination of antioxidant activity has two components: 1) a generator of free radicals of Reactive oxygen species, ROS, and 2) a detector allowing quantification of the generated species and indicating changes in the measured signal in response to the presence of antioxidative compounds. It is well recognized that acoustic cavitation in liquids give rise to very intense local physicochemical effects that could lead to the hemolytic cleavage of covalent bonds between the oxygen and hydrogen atoms comprising the water molecules present. As a result, when liquid is irradiated by an ultrasonic wave, water and oxygen molecules inside the bubble are dissociated and reactive radical species are created in the bubble. Thus, ultrasonic irradiation is considered an effective source for ROS generation. It was indicated in our previous study1,2) that the intensity of Luc2+ SCL is essentially due to the generation of O2•−in the ultrasonic fields. That is to say, the SCL system permits the quantification of antioxidant activity without using an enzyme for O2•− production. The potential use of SCL in determining antioxidant activity has not been well investigated, and published literature in this area is very scarce. In this study, the SCL of the Luc2+/2-propanol system is proposed for the evaluation of antioxidant activity against O2•− for the first time. The kinetic aspects of the SCL reactions are discussed.
