Poly (N-Isopropylacrylamide-co-2-hydroxyethyl methacrylate) (P(NIPAM-co-HEMA)) was synthesized using ultrasonic irradiation in a mixed solvent of water and ethanol without chemical initiator. The effects of ultrasonic intensity, irradiation time and monomer molar ratio on lower critical temperature (LCST) (measurement of responsive temperature) of synthesized polymers were investigated. The LCST of the copolymer obtained at the completion of the reaction linearly increased with the copolymer composition. However, the LCST of the copolymer obtained in the early stage of the reaction was higher than that estimated by the copolymer composition. These results suggested that some parts of the polymer chain have higher NIPAM composition than overall composition because of the difference of the reactivity of the monomers. The difference in the LCST between experimental data and estimated values from copolymer composition was higher for higher NIPAM ratio of initial monomer composition.