Abstract
Nano-structured titanium oxide layer was prepared on mirror polished titanium substrates by thermal oxidation at 600C for various periods ranging from 1 hour up to 24 hours in order to examine the effect of nanometer-scale surface roughness of titanium oxide layer on adhesion and proliferation of osteoblast-like cell;MC3T3-E1. TF-XRD analysis showed that rutile was formed on the titanium substrates and its crystallite size increased with the heat-treatment time. AFM analysis showed that surface roughness depended on the heat treatment time and the roughness average (Ra) was controlled in the range from 15 to 64 nm. The cell proliferation was remarkably increased on the substrate after being heated for 6 hours. It is concluded that cell proliferation is highly affected by nanometer-scale surface roughness.
