Efficiency of protein immobilization into an apatite layer using a supersaturated calcium phosphate solution -Influence of molecular weight and isoelectric point of protein-

Abstract

A protein-immobilized apatite layer can be formed in a supersaturated calcium phosphate solution (CP solution) supplemented with protein. Efficiency of protein immobilization (percentage of protein immobilized in the apatite layer among all the protein supplemented to the CP solution) was not correlated with molecular weight of the protein, but well correlated with isoelectric point of the protein. This might be because electrostatic interaction between protein and apatite crystal is crucial in formation of the protein-immobilized apatite layer in the CP solution.