Abstract
Cytochrome C (cyt C) was immobilized on a NaOH- and heat-treated titanium substrate (Ti substrate) in calcium phosphate (CaP) solutions with different initial supersaturation levels for one day at 25 °"C. Expectedly, the amounts of both immobilized cyt C and deposited calcium phosphate on the Ti substrate increased when the initial supersaturation level of the CaP solution was increased. However, the cyt C content in the certain amount of deposited calcium phosphate decreased with an increase in initial supersaturation level. This result means that the initial supersaturation level affected the density of the cyt C in the calcium phosphate layer.
