Formation of hydroxyapatite from calcium phenylphosphate by an enzymatic reaction

Abstract

The present study focused formation of hydroxyapatite (HAp) from calcium phenylphosphate (CaPP) in simulated body fluid (SBF) containing alkaline phosphatase (ALP). ALP acts as a catalyst to hydrolyze phosphate esters, and results in gradual formation of phosphate ions. The emitted orthophosphate ion reacted with calcium ion to form HAp crystals, which have scale-shaped crystals in SBF. Transformation rate of CaPP to HAp was increased with increasing ALP concentrations in SBF. The increased rate of transformation is attributed to enhanced hydrolysis of the phenyl phosphate by increasing with the amounts of ALP.