Abstract
Previous studies have reported positive reaction for Ki-67, and expression of osteoblastic markers, such as osteopontin and osteocalcin, in three-dimensional sheets of human alveolar bone periosteal cells (HABPCs). The purpose of the present study was to examine the possibility of use of these cells in periodontal regeneration therapy by observing the immunohistochemical changes in HABPC sheets transplanted into immunodeficient mice. One, two, four, and eight weeks after the transplantation, the animals were sacrificed and the process of angiogenesis and osteogenic potential were identified by the expression of blood vessel markers and bone markers. Two weeks after the transplantation, angiogenesis was observed inside/in the HABPCs sheets, which became more and more pronounced with increasing interval after the implantation Angiogenesis was three identified in hematoxylin and eosin-stained sections at two weeks, and became more pronounced with increasing interval from the transplantation. One week after the transplantation, ALP staining was positive, however, there was no reaction at eight weeks; von Kossa staining was positive at eight weeks after the transplantation. Positive reaction for OP, OC, Col - I and Runx-2 was found at two weeks after the transplantation, in addition to positive reaction for VEGF and FGF-2 at one week. Moreover, positive staining for VWF and CD31 was observed in the transplanted tissue, suggesting that the new blood vessels grew from the recipient mouse tissues. This study suggested that the periosteal cell sheets developed the ability for calcification following transplantation, and therefore appeared to have the potential for use in periodontal regeneration therapy.
Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 53(1) : 13-26,2011.
