Abstract
β-Glucuronidase (EC 3.2.1.31β-G), β-Acetylgalactosaminidase (EC 3.2.1.30.β-AG) and Acid phosphatase (EC 3.1.3.2AP) activities in whole saliva collected from patients with periodontal disease (patient group) and clinically healthy subjects (normal group) were measured by fluorometric assay, and were compared with some clinical findings (OHI-S, PMA-I, pocket depth and bone loss).
The following results were obtained;
1. β-G, β-AG and AP activities of patient group without Triton X-100 (TX) were 7.4, 13 and 3.6 times higher than those of normal group, and these enzyme activities of patient group with TX were 7.6, 15 and 4.6 times higher than those of normal group, respectively.
2. β-AG and AP activities with TX were average 2 and 21% higher than the ones without TX in normal group and also average 14 and 53% higher in patient group, respectively. However, β-G activity with TX was lower than the one without TX in both groups.
3. There were obsrerved significant correlations between each of these enzyme activities and some clinical findings, ie., pocket depth and bone loss (P<0.01-0.05).
