Abstract
The purpose of this study was to investigate the effect of specific antibody administration on the periodontium of Actinomyces viscosus Ny 1 R 2 infected rats.
Thirty-four rats after ablactation were divided into 3 groups. At 4 weeks of age, A. viscosus Ny 1 R 2 was inoculated in antibody-injected group and PBS-injected group. Non-injected group was not subjected to inoculation. All groups were fed powdered diet throughout experimental period.
At 3 month old, 0.4ml of anti-A. viscosus Ny 1 R 2 gamma-globulin was injected from tail vein once a day for 6 days in antibody-injected group. In PBSinjected group, phosphate buffered saline was injected in the same manner. No injection was carried out in non-injected group. After last injection, rats were sacrificed and the maxilla was excised. The left side was used for bacterial count and the right for histological evaluation.
Colony forming unit of A. viscosus Ny 1 R 2 was 8.50±0.20, 8.51±0.44 (logCFU) in antibody-injected group and PBS-injected group, respectively. In histological findings, polymorphonuclear leukocytes (PMNs) count in the epithelium of interdental papilla was 10.7±5.4, 23.2±27.6, 10.6±3.6 in antibodyinjected, PBS-injected and non-injected group, respectively.
A tendency for decrease in both standard deviation of CFU of A. viscosus Ny 1 R 2 and mean PMNs count was demonstrated in antibody-injected group compared to PBS-injected group, although no statistically significant difference was observed between two groups. It is suggested that antibody has a slight inhibitory effect on periodontal inflammation.
