1992 Volume 34 Issue 1 Pages 73-82
The purpose of the present study was to investigate the effect of human periodontal ligament fibroblasts and human gingival fibroblasts on bone resorption induced by LPS from Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Escherichia coli in an organ culture system using murine calvarial bones. These fibroblasts, at an initial density of 1. 105 cells/well, were seeded into 24-well plates with minimum essential medium alpha (αMEM) and 10% fetal bovine serum for 24 hr. Then, the cells were preincubated for 24 hr in αMEM. The calvarial bones were preincubated for 24 hr in αMEM. The preincubated calvarial bones were placed on filter membranes (Millicell -HA®). The fibroblasts were then co-cultured with the above calvarial bones. The medium which contained the calvarial bones and the fibroblasts was replaced with fresh medium containing 50μg/ml LPS. The medium which contained the calvarial bones alone was also replaced with fresh medium containing 50μg/ml LPS. They were then cultured for 5 days without changing the medium. The amount of calcium released into the medium was assessed by the Calcium C-Test (® Wako). In the present study, human periodontal ligament fibroblasts and human gingival fibroblasts inhibited bone resorption induced by LPS from Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Escherichia coli. Based on the results of the present study, it was suggested that these fibroblasts might play a role in the inhibition of bone resorption.
