The Effects of Bisphosphonates on Polymorphonuclear Leukocyte-Derived Matrix Metalloproteinase Activity
1999 Volume 41 Issue 2 Pages 99-107
Details
1999 Volume 41 Issue 2 Pages 99-107
This study investigated the effects of bisphosphonates on the polymorphonuclear leukocyte (PMN) -derived matrix metalloproteinases (MMPs) /MMP-8 and MMP-9 activity in vitro. PMNs extracts were treated with various concentrations (1× 10-6-1× 10-3M) of tiludronate, etidronate, and tetracycline. MMP-8 and MMP-9 activities were assayed using FITC-conjugated type I collagen and type IV collagen as substrate. MMP-8 activity was inhibited by 1× 10-6 M tiludronate and by 1× 10-3 M tetracycline, but not inhibited by etidronate. MMP-9 activity was inhibited by tiludronate, etidronate, and tetracycline in a dose-dependent manner. The results revealed that PMN-derived MMP activities were inhibited by bisphos-phonates. Especially, the inhibition of MMP activities by tiludronate was more evident than that by tetracycline, as is already well-known. In addition to a well-known pharmacological action, inhibition of osteoclastic bone resorption, the capability of bisphosphonates to inhibit the activities of PMN-derived MMPs may be beneficial to prevent tissue destruction in periodontal inflammation. J. Jpn. Soc. Peri-odontol., 41: 99-107, 1999.
