2001 Volume 43 Issue 3 Pages 251-259
We studied the amount of dead polymorphonuclear leukocytes (PMNs) in gingival clevicular fluid (GCF) under going apoptosis comparing gingiva in adult periodontitis patients and healthy controls. GCF PMNs (GC-PMNs) were collected from 5 patients with adult periodontitis and 5 controls. To study the effect on peripheral blood PMNs (PB-PMNs) death by GCF, we isolated PB-PMNs from controls. GCF supernatant was taken from healthy (H-GCF) and diseased (AP-GCF) gingiva. To identify apoptotic and necrotic cells, FITC-annexinV and propidium iodide were used with flow cytometry and confocal laser scanning microscopy. We found much higher rate of apoptotic (19.48±2.4%), and necrotic (16.79±4.8%) cells in AP-GCF; the rate of apoptotic and necrotic cells in H-GCF was 3.25±1.86% and 3.52±3.4%. Significantly fewer living cells were found in AP-GCF than in H-GCF. Comparisons among the rate of apoptotic cells of PB-PMNs stimulated by GCF showed controlled (3.87±1.1%) and AP-GCF (3.12±0.87%) to both be significantly higher than H-GCF (1.59±0.57%).
These results demonstrate that diseased sites contain GC-PMNs that under go eithe apoptosis or necrosis more readily than in healthy subjects. J. Jpn. Soc. Periodontol., 43:251-259, 2001
