Callus Induction in Rice, Oryza sativa L.
1967 年 43 巻 2 号 p. 156-160
詳細
1967 年 43 巻 2 号 p. 156-160
Callus induction in the rice plant, Oryza sativa L. var. Kyoto Asahi was tested by using completely synthetic media. Callus induction depends on the 2, 4-dichlorophenoxyacetic acid (2, 4-D) concentration only, and kinetin is not absolutely essential for callus induction. Callus was also formed on the medium including 2, 4-D and an adenine derivative 1-ethoxy-9-ethyladenine hydroiodide (Y-96). Shikimic acid had no affect on callus induction. The same concentration of indole-3-acetic acid (IAA) as that of 2, 4-D did not induce callus but at 100ppm callus induction occurred. Chromosomal aberration was observed in callus induced from roots and nodes of shoots. Frequency of aneuploid cells was 80% and 57% in calluses from roots and nodes respectively.
